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Please use this identifier to cite or link to this item: http://hdl.handle.net/2080/1065

Title: Optimization of Culture Medium for Production of Recombinant Dengue Protein in Escherichia Coli
Authors: Tripathi, N K
Shrivastva, A
Biswal, K C
P V, Lakshmana Rao
Keywords: Escherichia coli,
fed-batch cultivation,
recombinant dengue protein
Issue Date: 1-Sep-2009
Publisher: Mary Ann Liebert
Citation: Industrial Biotechnology. September 2009, Volume 5 No 3, pages 179-183
Abstract: Enhanced production of recombinant dengue multi-epitope protein in Escherichia coli was achieved by optimization of culture medium. Complex media (Luria Bertani broth, Terrific broth, Super broth, M9 minimal media, five times Luria Bertani broth, semi-defined medium enriched with tryptone and yeast extract, and semi-defined medium enriched with glucose) were evaluated for production of recombinant dengue multi-epitope protein in shake flasks. The recombinant protein was further produced by fed-batch fermentation using 5 L bioreactor. Cells were grown in optimized semi-defined medium, and feeding was carried out with 5X medium and glycerol. When growth reached 14.35 g/L of dry cell weight, culture was induced with 0.5 mM IPTG and further grown for 4 h to reach 18.37 g/L dry cell weight. The recombinant dengue multi-epitope protein was purified from inclusion bodies under denaturing conditions using metal affinity chromatography, which yielded 96.43 mg/L of protein. The purified protein ...
URI: http://dx.doi.org/10.1089/ind.2009.3.179.
http://hdl.handle.net/2080/1065
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