AtATG6-Mediated Cell Death in Arabidopsis Confers Immunity Against Magnaporthe oryzae

Abstract

Magnaporthe oryzae can infect rice, however, Arabidopsis is a nonhost that actively fights the infection. Despite the discovery of numerous genes and QTLs, rice blast has not been cured. In order to protect Arabidopsis against rice blast, the non-host resistance (NHR) mechanism triggers the hypersensitive response (HR), a type of localized programmed cell death associated with pathogen limiting or killing that results in resistance to pathogenic infections. Cell death is generally thought to be linked to disease resistance in a physiological and genetic way. We examined the connection between AtATG6 and HR cell death, emphasizing the part that cell death plays in the emergence of M. oryzae disease resistance. M. oryzae was used to challenge the wild-type Col-0 and mutant atg6 in order to examine the infection dynamics and cell survival using trypan blue staining and electrolyte assay. To further examine the connection between ROS production and cell death, the comparative oxidative burst was visualized under a microscope. According to the relative expression patterns of the defense genes PR1, WRKY53, and WRKY29, the mutant works by undermining defense. Plant immunity was compromised, as evidenced by the Raman spectra study, which caused the treated plant's carotenoid levels to differ from the control.