Bioengineered Biohybrid Composite for Controlled Drug Release

Abstract

Advances in pharmacokinetics demonstrated the significance of drug release in favoring therapeutic effectiveness. Developing a dynamic cascade drug delivery system facilitates prolonged drug release and personalized treatment. In our present study, Piperineloaded xenogenic pericardium scaffold properties are evaluated for a potential biohybrid-based drug delivery system. Decellularized tissues are utilized as implanted drug delivery devices to extend the supply of medications and genetic materials over a set period of time to avoid postsurgery immune response and other chronic conditions. Piperine, the pungent component in black pepper (Piper nigrum L.), exhibits various pharmacological effects, including antitumor and antioxidant activities. Incorporating the aforesaid compound into the matrix is a potential biohybrid scaffold with sustained drug release. Piperine is extracted from Piper nigrum dry seeds using the ethanol extraction method. The caprine pericardium is decellularized by SDS and Triton X-100. A biohybrid of decellularized caprine pericardium-piperine is prepared using a solvent evaporation method. The physicochemical properties of Piperine, the decellularized tissue, and the fabricated biocomposite are investigated. The swelling and drug release for the drug-loaded matrix is performed. The swelling studies revealed a decrease in the swelling percentage of Piperine-loaded decellularized pericardium (199 ±29.78%) compared with the unloaded matrix. Piperine-loaded decellularized pericardium and the prospective use of a natural scaffold is a promising tool in tissue engineering and regenerative medicine.