Optimization and Characterization of Detoxified Cassava (Manihot Esculenta L.) Leaf Protein Isolate by Alkalin.E Extraction-Isoelectric Precipitation

Abstract

The cassava leaves protein isolate extraction and optimization were investigated using response surface methodology, where the maximum protein content (21.83±0.41 g/100g dm), extraction yield (18.31±0.53%), and protein recovery yield (69±1.31%) were obtained at optimal conditions: 114min extraction time, 46◦C extraction temperature, 23.5mL/g solvent/solute ratio and pH 11.0 value. The presence of toxicant (Cyanide) and anti-nutrient (tannin) in cassava leaves reduced the bio-accessibility of its protein isolate, strictly prohibiting its consumption. Therefore, detoxification was applied to diminish cyanide and tannin to 85% and 69% in leaves, respectively, where the protein content was reduced to 9.7%. However, detoxified cassava leaf protein isolate exhibited changes in the compositional, structural, morphological, molecular, and thermal characteristics compared to controlled one. Moreover, the functional properties in protein isolate improved after detoxification at different pH conditions, which can be used as an active ingredient in various foods