Mass Synthesis of Bacoside A and Biomass from Bacopa Monnieri Cell Suspension Culture Using a Bioreactor

Abstract

Bacopa monnieri; also known as ‘Brahmi', is one of the most important Indian medicinal herbs. The main bioactive compounds in brahmi are saponin called as bacoside A. Bacoside content in field grow plant ranges from 0.05%-0.85%. Bacoside is a key ingredient in many traditional medicine formulations that target CNS and tackle disorder consisting of memory loss, and anxiety. These compounds are naturally synthesized in quite a small quantity, therefore purification from biological material gives rise to a limited yield, and impurities and utilizes an exceptionally huge measure of biomass. Bioreactors are effective alternatives to traditional crop production due to their stability, improved regulations of culture conditions, more effective nutrient uptake capacity, huge amounts of biomass, and product outcome. In this current study, callus culture from leaf explant was established and presence of bacoside was confirmed using HPLC. Culture condition for callus suspension culture using shake flask was optimized using various statistical tool such as Plackett-Burman and Response Surface Methodology to determine maximum biomass, cell viability and bacoside content. Mass synthesis was carried out from cell suspension culture using various mode of bioreactor (batch and fed batch strategy) where intermittent sampling was performed to determine substrate consumption, biomass and bacoside.