Effect of Carbon Sources and Dye Degradation On Growth Profile and Lipid Production of Rhodosporidium Paludigenum

Abstract

Industries like textile, dye manufacturing, painting, printing, food, pharmaceutical, and cosmetics release many xenobiotic compounds like dye (Congo red, Methylene blue, and Crystal violet). The effects of carbon sources and dye on the growth, dry biomass, and lipid production of R. paludigenum has been studied. The carbon sources used for the study were glucose, sucrose, fructose, maltose, lactose, and xylose. The growth profile of R. paludigenum was studied in simulated wastewater (MSM) containing carbon sources for an incubation period of 144. The log phase started from 12 h of incubation and lasted up to 108 h, but xylose and maltose have shorter log phases, i.e., 48 h. R. paludigenum grows in MSM media containing these carbon sources and produces the maximum biomass and lipid at 72 h of incubation, i.e., glucose>fructose>sucrose>lactose>maltose>xylose respectively. The maximum and minimum biomass(g/l) and lipid (%) produced by glucose (7,55) and xylose (4.5,26) respectively. The effect of dye on growth, biomass, and lipid production was studied. The results confirmed that R. paludigenum utilizes 25 ppm Methylene blue dye more efficiently and produces a maximum biomass 3.27 g/L and lipid content 30%, respectively. Therefore, R. paludigenum can be used to reclamation wastewater and produce green fuel.