Modulation of Biosynthesis of Bacosides by Cell Suspension Culture of Bacopa Monnieri in Novel Bioreactor

Abstract

Bacosides are pharmaceutically important compounds that have immense importance for the treatment of memory disorders and Alzheimer’s disease. At NIT Rourkela we attempted to perform initial docking simulations using the molecular structure of Bacoside A and other phytopharmaceuticals and commercial drug for the inhibition of acetyl cholinesterase. The results suggested bacoside A showed good anti-Alzhiemer’s drug properties like high binding energy value, good drug likeness property and group 4 toxicity. Central composite design was utilized to predict the best model for prediction of optimal production of bacosides in cell suspension cultures. The model predicted values were compared with experimental values for biomass production and bacoside A concentration in cell suspension cultures which showed high correlation for both the parameters. The bioprocess strategy was also optimized systematically for optimal callus induction, followed by callus proliferation. The kinetics of growth and product formation were studied under optimized conditions to find the values of kinetic parameters under experimental conditions. The production of bacoside A was mainly non-growth associated and found to be maximum during the stationary phase of the cell suspension culture. The production of bacosides was scaled up to 5-l stirred tank bioreactor in the batch and repeated batch mode. The production of bacosides was much higher in the repeated batch mode. These bioprocess strategies can be helpful for the enhanced production of various other valuable triterpenoid saponins.