Please use this identifier to cite or link to this item:
http://hdl.handle.net/2080/3794
Title: | Process optimization and production of Polyhydroxyalkanoates from sugarcane molasses by Pseudomonas sp. PhNs10. |
Authors: | Patil, Pritam Bajirao Sarkar, Debapriya Poddar, Kasturi Sarkar, Angana |
Keywords: | Polyhydroxyalkanoates Virulence Pseudomonas Sugarcane molasses |
Issue Date: | Nov-2022 |
Citation: | International Conference on Challenges in Environmental Science & Engineering CESE-2022, Dubai, 27 Nov-01 Dec 2022 |
Abstract: | Replacing synthetic plastics with eco-friendly alternatives like polyhydroxyalkanoates is a feasible option to reduce the level of plastic pollution. A non-virulent strain isolated from the soil of a local plastic waste disposal site was studied for the production of PHA in glucose as well as sugarcane molasses media. The 16S rRNA identification confirmed that the strain was related to the Pseudomonas genus. The carbohydrate utilization study revealed the ability of the strain to utilize different carbohydrates. The PHAs produced in both media were compared for their chemical and thermal properties using NMR, FTIR, Raman spectroscopy, contact angle measurement, and DSC. The production process in both media was optimized using RSM and the optimized conditions for glucose medium were found to be glucose and yeast extract concentrations of 3.33% and 0.33% respectively, pH of 7.11, and incubation time of 70 h. While the optimized conditions for molasses medium were found to be molasses concentration of 6.18%, pH 7.24, Inoculum percentage of 4.05%, and incubation time of 85 h. The growth, substrate utilization, and production kinetics study of Pseudomonas sp. PhNs10 revealed a specific growth rate of 0.07 and 0.04/h, specific biomass productivity of 0.06 and 0.04 g/L.h, and specific PHA productivity was 0.04 and 0.01g/L.h for glucose and molasses medium respectively. The yields of PHA from biomass were found to be 0.62 and 0.55 g of PHA per g of biomass for glucose and molasses medium respectively. The yield of PHA from glucose and molasses medium were found to be comparable and these findings could aid in the economic production of PHA |
Description: | Copyright belongs to proceeding publisher |
URI: | http://hdl.handle.net/2080/3794 |
Appears in Collections: | Conference Papers |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
SarkarA_CESE2022.pdf | 3.73 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.