Please use this identifier to cite or link to this item:
http://hdl.handle.net/2080/1065
Title: | Optimization of Culture Medium for Production of Recombinant Dengue Protein in Escherichia Coli |
Authors: | Tripathi, N K Shrivastva, A Biswal, K C P V, Lakshmana Rao |
Keywords: | Escherichia coli, fed-batch cultivation, recombinant dengue protein |
Issue Date: | 1-Sep-2009 |
Publisher: | Mary Ann Liebert |
Citation: | Industrial Biotechnology. September 2009, Volume 5 No 3, pages 179-183 |
Abstract: | Enhanced production of recombinant dengue multi-epitope protein in Escherichia coli was achieved by optimization of culture medium. Complex media (Luria Bertani broth, Terrific broth, Super broth, M9 minimal media, five times Luria Bertani broth, semi-defined medium enriched with tryptone and yeast extract, and semi-defined medium enriched with glucose) were evaluated for production of recombinant dengue multi-epitope protein in shake flasks. The recombinant protein was further produced by fed-batch fermentation using 5 L bioreactor. Cells were grown in optimized semi-defined medium, and feeding was carried out with 5X medium and glycerol. When growth reached 14.35 g/L of dry cell weight, culture was induced with 0.5 mM IPTG and further grown for 4 h to reach 18.37 g/L dry cell weight. The recombinant dengue multi-epitope protein was purified from inclusion bodies under denaturing conditions using metal affinity chromatography, which yielded 96.43 mg/L of protein. The purified protein was found to be reactive with dengue-infected human serum samples |
URI: | http://dx.doi.org/10.1089/ind.2009.3.179. http://hdl.handle.net/2080/1065 |
Appears in Collections: | Journal Articles |
Files in This Item:
File | Description | Size | Format | |
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Ind Biotech paper.pdf | 1.32 MB | Adobe PDF | View/Open |
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