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http://hdl.handle.net/2080/1065
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DC Field | Value | Language |
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dc.contributor.author | Tripathi, N K | - |
dc.contributor.author | Shrivastva, A | - |
dc.contributor.author | Biswal, K C | - |
dc.contributor.author | P V, Lakshmana Rao | - |
dc.date.accessioned | 2009-11-04T07:57:04Z | - |
dc.date.available | 2009-11-04T07:57:04Z | - |
dc.date.issued | 2009-09-01 | - |
dc.identifier.citation | Industrial Biotechnology. September 2009, Volume 5 No 3, pages 179-183 | en |
dc.identifier.uri | http://dx.doi.org/10.1089/ind.2009.3.179. | - |
dc.identifier.uri | http://hdl.handle.net/2080/1065 | - |
dc.description.abstract | Enhanced production of recombinant dengue multi-epitope protein in Escherichia coli was achieved by optimization of culture medium. Complex media (Luria Bertani broth, Terrific broth, Super broth, M9 minimal media, five times Luria Bertani broth, semi-defined medium enriched with tryptone and yeast extract, and semi-defined medium enriched with glucose) were evaluated for production of recombinant dengue multi-epitope protein in shake flasks. The recombinant protein was further produced by fed-batch fermentation using 5 L bioreactor. Cells were grown in optimized semi-defined medium, and feeding was carried out with 5X medium and glycerol. When growth reached 14.35 g/L of dry cell weight, culture was induced with 0.5 mM IPTG and further grown for 4 h to reach 18.37 g/L dry cell weight. The recombinant dengue multi-epitope protein was purified from inclusion bodies under denaturing conditions using metal affinity chromatography, which yielded 96.43 mg/L of protein. The purified protein was found to be reactive with dengue-infected human serum samples | en |
dc.format.extent | 1355965 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | Mary Ann Liebert | en |
dc.subject | Escherichia coli, | en |
dc.subject | fed-batch cultivation, | en |
dc.subject | recombinant dengue protein | en |
dc.title | Optimization of Culture Medium for Production of Recombinant Dengue Protein in Escherichia Coli | en |
dc.type | Article | en |
Appears in Collections: | Journal Articles |
Files in This Item:
File | Description | Size | Format | |
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Ind Biotech paper.pdf | 1.32 MB | Adobe PDF | View/Open |
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